
Raman Spectroscopy for Surgical Margins: Intraoperative Guide
Raman spectroscopy for surgical margin assessment produces diagnostic-quality tissue images in under 3 minutes - clinical trials validate real-time use.

Integrate Horiba, Renishaw, and Thermo Fisher Raman spectrometers into clinical diagnostic workflows. SpectraDx delivers instrument automation, spectral classification, and seamless EHR integration for Raman-based diagnostics.
Overview
Raman spectroscopy works by shining a laser — typically green at 532 nm — at a sample and measuring the scattered light. Most photons scatter elastically at the same energy, but a small fraction scatter inelastically, shifted by an amount that corresponds to specific molecular vibrations. This Raman shift creates a molecular fingerprint complementary to FTIR and often more specific.
Clinically, Raman has distinct advantages: it works through water (unlike FTIR), requires minimal sample preparation, and can analyze samples through glass or plastic containers. These properties make it ideal for point-of-care applications — bacterial identification directly from clinical specimens, real-time drug identification in emergency departments, and intraoperative tissue assessment during surgery.
The instrumentation is mature. What's missing is the software layer that transforms Raman measurements into clinical actions — patient association, quality control, ML-based classification, and structured reporting to hospital information systems.
Bacterial ID & AST
Identify pathogens and predict antibiotic resistance in under 90 minutes — versus 24–72 hours for culture
Surgical Margin Assessment
Real-time tissue classification during surgery using stimulated Raman histology (SRH)
Drug Identification
Identify unknown substances including fentanyl analogs through sealed packaging in the ED
SERS Diagnostics
Surface-enhanced Raman for ultra-sensitive point-of-care detection of biomarkers and pathogens
Instruments
SpectraDx provides modular instrument adapters. Each adapter handles vendor-specific protocols so your clinical workflow stays consistent.
Horiba
LabSpec SDK integration for confocal Raman mapping, point measurements, and automated spectral acquisition.
Horiba
Compact research-grade Raman with motorized stage and LabSpec automation interface.
Renishaw
WiRE software integration for high-performance confocal Raman with LiveTrack focus tracking.
Renishaw
Fiber-optic coupled Raman analyzer designed for remote and clinical sampling applications.
Thermo Fisher
OMNIC integration for the DXR3 Smart Raman with automated alignment and fluorescence correction.
Forward Edge AI
Handheld Raman with built-in ML classification and cloud connectivity for field and clinical use.
The Problem
Instrument vendor software like LabSpec (Horiba), WiRE (Renishaw), OMNIC (Thermo Fisher) is designed for research, not clinical deployment.
Platform
Programmable measurement sequences for Horiba and Renishaw spectrometers. Automated laser power, exposure time, and accumulation settings for consistent clinical results.
Learn more →Deploy ML models trained on Raman spectra for bacterial identification, tissue classification, and substance identification with calibrated confidence scores.
Learn more →Route Raman diagnostic results through HL7v2 or FHIR R4 to your LIS/EHR. Support for LOINC observation codes and CPT billing.
Learn more →Process surface-enhanced Raman spectra from nanostructured substrates for high-sensitivity point-of-care diagnostic applications.
Learn more →Resources
In-depth technical articles for Raman integration, clinical workflows, and regulatory compliance.
From Raman spectra to clinical results in your EHR. Bacterial ID, drug testing, and surgical guidance — one platform.